Folate content in sea buckthorn berries and related products (Hippophae rhamnoides L. ssp rhamnoides): LC-MS/MS determination of folate vitamer stability influenced by processing and storage assessed by stable isotope dilution assay

  • 文献类型:   Article
  • 作  者:   GUTZEIT D, MONCH S, JERZ G, WINTERHALTER P, RYCHLIK M
  • 关键词:   folate, stable isotope dilution assay, lcesims/ms, hippophae rhamnoide, sea buckthorn product, process storage stability
  • 出版物名称:   ANALYTICAL BIOANALYTICAL CHEMISTRY
  • ISSN:   1618-2642
  • 通讯作者地址:   Tech Univ Munich
  • 被引频次:   15
  • DOI:   10.1007/s00216-008-1905-3
  • 出版年:   2008
  • 研究对象:   药用植物
  • 研究主题:   分离合成测定

▎ 摘  要

A stable isotope dilution assay was adopted for quantitation of folate vitamers in sea buckthorn berries, juice, and concentrate using fourfold labeled folate isotopologues of the folate derivatives as the internal standards and reversed-phase liquid chromatography-tandem mass spectrometry with electrospray ionization (LC-ESI-MS/MS). Processing effects and storage stability were investigated during juice and concentrate production from sea buckthorn berries (Hippophae rhamnoides). The technological processing of the berries caused a total degradation of tetrahydrofolate and 5-formyltetrahydrofolate in the generated juice. The content of the main folate vitamer 5-methyltetrahydrofolate remained approximately unchanged during the whole processing from the berries to the concentrate. Sea buckthorn juice was stored under two household storage conditions (6 degrees C, 25 degrees C), and also under accelerated aging conditions (40 degrees C) for up to 7 days to determine the effects of storage temperature on the stability of 5-methyltetrahydrofolate. The content of 5-methyltetrahydrofolate was nearly unchanged during the storage at 6 degrees C after 7 days. The juice showed almost identical degradation of 5-methyltetrahydrofolate of about 17-20% at 25 degrees C and 40 degrees C after 7 days of storage.

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